Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anticancer effects of Mori radicis cortex, Mori ramulus, Angelica tenuissima, and Inonotus obliquus extract mixtures on B16F10 murine melanoma cells via up-regulation of p53 and down-regulation of Akt expression

Kyeng Min Kim¹, Eunhee Choi², Jung Hyun Lee³, Kukdong Kim¹, Prabhat Kumar Mandal⁴, Kangduk Choi^1,5

¹Department of Biotechnology, Hankyong National University, Anseong-si, 17579, China; ²Kwanghye Hospital, Seoul 06174, South Korea; ³Public Health Care of Yeongyang-gun, Ministry of Health and Welfare of South Korea, Yeongyang-gun 36540, Republic of Korea; ⁴Department of Livestock Products Technology, Rajib Gandhi Institute of Veterinary Education and Research, Pondicherry, India 605 009; ⁵Genomic Informatics Center, Hankyong National University, Anseong-si 17579, Republic of Korea.

For correspondence:- Kangduk Choi Email: kchoi04@hknu.ac.kr Tel:+82316705422

Accepted: 24 January 2023 Published: 27 February 2023

Citation: Kim KM, Choi E, Lee JH, Kim K, Mandal PK, Choi K. Anticancer effects of Mori radicis cortex, Mori ramulus, Angelica tenuissima, and Inonotus obliquus extract mixtures on B16F10 murine melanoma cells via up-regulation of p53 and down-regulation of Akt expression. Trop J Pharm Res 2023; 22(2):277-282 doi: 10.4314/tjpr.v22i2.8

© 2023 The authors.
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Abstract

Purpose: To investigate the anticancer effects of the extract mixture of Mori radicis cortex, Mori ramulus, Angelica tenuissima, and Inonotus obliquus extract (MRME), a new herbal preparation from Korea, on B16F10 cells.

Methods: The MRME was extracted with water. Cell Counting Kit-8 (CCK-8) assays and 4′,6- diamidino-2-phenylindole (DAPI) staining were used to investigate the effect of MRME on cell proliferation and apoptosis in vitro. The expression of p53, p-p53, and phosphorylated Akt (p-Akt) were analyzed by western blotting. Wound-healing assay was used to analyze cell migration.

Results: Based on CCK-8 and DAPI staining results, MRME inhibited the growth of B16F10 cells and induced apoptosis in B16F10 cells in a time- and dose-dependent manner (p < 0.05). MRME increased the expression of p53 and p-p53 and decreased the expression of p-Akt in a dose-dependent manner. MRME treatment inhibited cell migration and invasion (p < 0.05).

Conclusion: Mori Radicis Cortex Mixture Extract exerts anticancer effect by inducing the expression of p53 and p-p53 and decreasing the expression of p-Akt, which may benefit patients with malignant melanoma when used in combination with v-raf murine sarcoma viral oncogene homolog B1 (BRAF) inhibitors.

Keywords: Mori Cortex Radicis, Ramulus Mori, Angelica tenuissima, Inonotus obliquus, Melanoma, Anticancer, p53, Akt